Document Details
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Document Type |
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Thesis |
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Document Title |
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Cytological and Molecular Studies of some Psychotherapy Drugs Effect on Vicia faba root tips meristems and Saccharomyces cerevisiae. دراسات وراثية خلوية وجزيئية لتأثيرات بعض عقاقير العلاج النفسي على القمم النامية لجذور نبات الفول وخميرة سكاروميسس سيرفيسي |
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Subject |
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Genetics |
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Document Language |
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Arabic |
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Abstract |
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Many manufactured medicines and chemical drugs are considered to be the most important affections of the environment which humans can get exposed to, because these medicines and drugs have many complications and harmful side effects.
This study is aimed to cytological and biochemical study of three drugs used in psychotherapy and anti-depression treatment. These drugs are: Risperdal, Cymbalta and Faverin, to determine its toxic effects on Mitotic Index in root tips cells of Vicia faba plant, chromosome abnormalities, and to study the changes in protein bands patterns and DNA, in addition to determine the effects of these drugs on the survival rates of Saccharomyces cerevisiae (D7 strain) and its capabilities in making gene conversion ,reverse mutation and mitotic crossing over, and to study the changes in protein bands patterns, and DNA.
Results shown that the treatments with these drugs have led to:
1/The unbalance of the different mitotic stage rates compared to Control.
2/Decreasing of different mitotic index ratios compared with Control.
3/increasing of mitotic abnormalities ratios, which have appeared in all mitotic stages.
Result also shown that the most frequent abnormalities were:Stickness, disturbance, C-metaphase, abnormal prophase, bridges, unoriented chromosomes and unequal separation of chromosomes. Also various abnormalities have appeared in smaller rates like: Binucleated cell, polyploidy, fragments and breaks.
The different treatments with drugs led to the appearance of many changes in electrophoretic protein band patterns, such as changes in bands density, appearance of new protein bands, disappearance of protein bands, compared to Control.
Treatment with high dosage of Cymbalta drug led to changes in DNA band patterns such as:
* Disappearance of two bands.
* Did not lead to the appearance of new bands.
Treatment with high dosages of Risperdal and Faverin did not show any changes in DNA bands compared to Control.
Results have shown that all combined treatments with the drugs led to:
* Decrease the survival retes of Saccharomyces cerevisiae.
The drugs also showed capability to mutation induction such as: gene conversion, revers mutation and mitotic crossing over compared with their respective Control.
Also the treatments led to the appearace of changes in electrophoretic protein band patterns such as :
Changes inbands density, disappearance of protein bands and appearance of new bands, compared to Control.
Treatments with high dosages of the drugs led to changes in DNA band patterns such as: the disappearance of bands and the appearance of new bands compared to Control.
The results of the treatments showed the toxic effects of the drugs used in the study. The toxic effect of the different dosages of the drugs and exploring durations was more obvious on the Saccharomyces cerevisiae than on the Vicia faba root tips cells. |
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Supervisor |
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Dr. Jamal Sabir Mohamed Sabir |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1432 AH
2011 AD |
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Number Of Pages |
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234 |
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Added Date |
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Wednesday, May 11, 2011 |
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Researchers
| منى عبدالرحمن راعي | Raee, Mona Abdul-Rahman | Researcher | Doctorate | mraee@kau.edu.sa |
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